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Autofluorescence is generally the background signal that researchers try to remove. On the contrary, this was the very signal that my graduate work sought to maximize. Our bodies have the natural ability to emit light under discrete wavelengths. Certain molecules, such as chromophores and fluorophores, absorb and/or emit light. This is the premise for which autofluorescence may be applied to disease diagnostics, removing challenges that exogenous agents present.
Video courtesy of Dr.s Rajesh Raman and Chad Lieber.
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Video: Time-lapse autofluorescence images of rat kidney tubules acquired at 355 nm excitation. The tubules are refilling with blood after clamping. Reperfusion is seen as dynamic contrast differentials due to varying concentrations of circulating NADH.
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In order to translate my graduate work proof of concept from the lab to the clinic, I designed, built, and validated a portable Bench-top Endomicroscope for Visualization and Imaging of Nuclei.
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Ph.D. dissertation overview:
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M.S. thesis overview:
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